Efek Pemberian Monosodium Glutamat (MSG) Terhadap Morfologi Spermatozoa Epididymis Mencit (Mus musculus, L.)

Abstrak

This study aims to determine the effect of giving monosodium glutamate (MSG) on the morphology of epididymal spermatozoa in mice (Mus musculus, L.). A total of 16 male mice weighing 20-30 grams aged 2-3 months were divided into 4 treatments, namely 1 control (PAM water), and 3 treatments of monosodium glutamate, namely; T1 (1.5 ml/g BW), T2 (3.0 ml/g BW), and T3 (4.5 ml/g BW). MSG was administered orally for 34 days. On the 35th day, the mice's body weight was weighed, then dissected and the cauda epididymis was taken to observe spermatozoa morphology using the smear preparation method. Data were analyzed using ANOVA and further HSD Turkey test with a confidence level of 95%. The results showed that administration of monosodium glutamate (MSG) caused abnormal spermatozoa morphology and increased their percentage. The average percentage reduction in normal spermatozoa morphology in all treatments sequentially, namely the dose of 1.5 ml/g BW (T1) was 40.50%, the dose 3.0 ml/g BW (T2) was 55.42%, the dose was 4.5 ml /g BW (T3) was 74.75%, while the decrease was only 11.50% for the control (T0). Based on the research results, it was concluded that administration of monosodium glutamate (MSG) increased the percentage of abnormal epididymal spermatozoa in mice (Mus musculus, L.).

Key words: Monosodium glutamate, spermatozoa morphology, Mus musculus

Abstrak

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian monosodium glutamat (MSG) terhadap morfologi spermatozoa epididimis pada mencit (Mus musculus, L.). Sebanyak 16 ekor mencit jantan dengan berat badan 20-30 gram umur 2-3 bulan dibagi menjadi 4 perlakuan yaitu 1 kontrol (air PAM), dan 3 perlakuan pemberian monosodium gutamat yaitu; P1 (1,5 ml/g BB), P2 (3,0 ml/g BB), dan P3 (4,5 ml/g BB). MSG diberikan secara oral selama 34 hari. Pada hari ke-35 mencit ditimbang berat badannya, kemudian dibedah dan diambil cauda epididimisnya untuk diamati morfologi spermatozoanya dengan metode sediaan apus. Data dianalisis menggunakan ANOVA dan dilanjutkan uji HSD Turkey dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian monosodium glutamat (MSG) menyebabkan morfologi spermatozoa abnormal dan meningkatkan persentasenya. Rata-rata persentase penurunan morfologi spermatozoa normal pada semua perlakuan secara berurutan yaitu dosis 1,5 ml/g BB (T1) sebesar 40,50%, dosis 3,0 ml/g BB (T2) sebesar 55,42%, dosis 4,5 ml/g BB (T3) sebesar 74,75%, sedangkan penurunannya hanya sebesar 11,50% pada kontrol (T0). Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian monosodium glutamat (MSG) dapat meningkatkan persentase spermatozoa epididimis abnormal pada mencit (Mus musculus, L.).

Kata kunci : Monosodium glutamat, morfologi spermatozoa, Mus musculus,