Uji Aktivitas Antihiperurisemia Ekstrak Etanol Daun Hantap (Sterculia oblongata R. Brown) pada Mencit (Mus musculus L.) Hiperurisemia

Abstract

This study aims to determine the antihyperuricemia activity of the ethanol extract of hantap leaves (Sterculia oblongata R. Brown) in hyperuricemia mice (Mus musculus L.). This research is an experimental research which was arranged based on Completely Randomized Design (CRD). The data obtained were analyzed statistically using ANOVA (analysis of variance) (α = 0.05) and continued with the BNT test with a 95% confidence level. A total of 24 mice were divided into 6 treatments with each treatment consisting of 4 mice. Each treatment was started with standard feeding for 7 days and then the initial blood uric acid levels were measured. Furthermore, all test animals in each treatment (except the first treatment as a control treatment), were given potassium oxonate for 2 days until they reached hyperuricemia conditions, then one treatment was given allopurinol (positive control) and three treatments were given hantap leaf ethanol extract (concentration 1%, 4%, and 8%) for 14 days, then fasted for 1 day and the final uric acid level was measured. The results showed that the ethanol extract of hantap leaves had an effect on reducing uric acid levels in mice. Treatment with 1% extract decreased uric acid levels in mice by 1.125 mg/dL, treatment with 4% extract concentration decreased uric acid levels in mice by 1.075 mg/dL and treatment with 8% extract concentration reduced mice uric acid levels by 0.975 mg/dL.

Keywords: Hyperuricemia, Mice, Hantap Leaves, Potassium Oxonate, Allopurinol.

ABSTRAK

Penelitian ini bertujuan untuk mengetahui aktivitas antihiperurisemia ekstrak etanol daun hantap (Sterculia oblongata R. Brown) pada mencit (Mus musculus L.) hiperurisemia. Penelitian ini merupakan penelitian eksperimental yang disusun berdasarkan Rancangan Acak Lengkap (RAL). Data yang diperoleh dianalisis secara statistik menggunakan ANOVA (analysis of variance) (α = 0,05) dan dilanjutkan dengan uji BNT dengan taraf kepercayaan 95%. Sebanyak 24 ekor mencit dibagi dalam 6 kali perlakuan dengan masing-masing perlakuan terdiri dari 4 ekor mencit. Masing-masing perlakuan diawali dengan pemberian pakan standard selama 7 hari lalu diukur kadar asam urat darah awal. Selanjutnya, semua hewan uji pada setiap perlakukan (kecuali perlakukan pertama sebagai kontrol perlakuan), diberikan kalium oksonat selama 2 hari hingga mencapai kondisi hiperurisemia, selanjutnya satu perlakuan diberi allopurinol (kontrol positif) dan tiga perlakuan diberikan ekstrak etanol daun hantap (konsentrasi 1%, 4%, dan 8%) selama 14 hari, kemudian dipuasakan selama 1 hari dan diukur kadar asam urat akhir. Hasil penelitian menunjukkan bahwa ekstrak etanol daun hantap berpengaruh terhadap penurunan kadar asam urat mencit dengan dengan perlakuan ekstrak 1% menurunan kadar asam urat mencit dari 2,04 mg/mL menjadi 1,125 mg/dL, perlakuan konsentrasi ekstrak 4% menurunan kadar asam urat mencit dari 2,15 mg/dL menjadi 1,075 mg/dL dan perlakuan dengan konsentrasi ekstrak 8% menurunkan kadar asam urat mencit dari 2,175 menjadi 0,975 mg/dL.

Kata Kunci : Hiperurisemia, Mencit, Daun Hantap, Kalium Oksonat, Allopurinol.