Efek Antifertilitas Ekstrak Batang Brotowali (Tinospora crispa L.) Terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) dalam Upaya Pengendalian Hama Rodentia
Abstract
This study aims to determine the antifertility effect of Tinospora crispa L. stem extract on spermatozoa quality in the form of motility and morphology of mouse spermatozoa (Mus musculus L.). 20 male mice weighing 30-40 grams, aged 2-3 months, were divided into 5 treatments, namely; control (K1), placebo Na CMC 0.5% (K2), concentration of 0.05 g/g BW (K3), concentration of 0.06 g/g BW (K4), and concentration of 0.07 g/g BW (K5) Tinospora crispa L. stem extract. The extract was given orally every day for 34 days. On the 35th day, the body weight of the mice was weighed, and then sacrificed by anesthetizing with 10% chloroform, then dissected and the cauda epididymis was taken to observe the motility and morphology of mouse spermatozoa. Data were analyzed by ANOVA and BNT follow-up test (α = 0.05). The results showed that the administration of brotowali stem extract significantly reduced the motility and morphology of normal spermatozoa in mice at all concentrations. The highest decrease in motility was in the K4 (60.75%) and K5 (82.75%) treatments compared to the K1 (11%) treatments. K2 (16%) and K3 (16.25%). Brotowali stem extract also significantly reduced the percentage of normal spermatozoa morphology, namely K1 (19.25%), K2 (24.75%), K3 (45%), K4 (59%) and K5 (76.75%). The conclusion of this study is that brotowali stem extract can be used to control rodent pests biologically because it has an antifertility effect, namely reducing the quality of spermatozoa.
Key words: antifertility, Zingiber officinale, Mus musculus, motility, morphology, spermatozoa
This study aims to determine the antifertility effect of Tinospora crispa L. stem extract on spermatozoa quality in the form of motility and morphology of mouse spermatozoa (Mus musculus L.). 20 male mice weighing 30-40 grams, aged 2-3 months, were divided into 5 treatments, namely; control (K1), placebo Na CMC 0.5% (K2), concentration of 0.05 g/g BW (K3), concentration of 0.06 g/g BW (K4), and concentration of 0.07 g/g BW (K5) Tinospora crispa L. stem extract. The extract was given orally every day for 34 days. On the 35th day, the body weight of the mice was weighed, and then sacrificed by anesthetizing with 10% chloroform, then dissected and the cauda epididymis was taken to observe the motility and morphology of mouse spermatozoa. Data were analyzed by ANOVA and BNT follow-up test (α = 0.05). The results showed that the administration of brotowali stem extract significantly reduced the motility and morphology of normal spermatozoa in mice at all concentrations. The highest decrease in motility was in the K4 (60.75%) and K5 (82.75%) treatments compared to the K1 (11%) treatments. K2 (16%) and K3 (16.25%). Brotowali stem extract also significantly reduced the percentage of normal spermatozoa morphology, namely K1 (19.25%), K2 (24.75%), K3 (45%), K4 (59%) and K5 (76.75%). The conclusion of this study is that brotowali stem extract can be used to control rodent pests biologically because it has an antifertility effect, namely reducing the quality of spermatozoa.
Key words: antifertility, Zingiber officinale, Mus musculus, motility, morphology, spermatozoa
