Skrining Bakteri Endofit dari Daun Jeruju (Acanthus ilicifolius L.) Penghasil Antibiotik

Abstract

This study aims to isolate endophytic bacteria from sea holly leaves (Acanthus ilicifolius L.) and determine their antibacterial activity against two indicator bacteria, namely Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 35218. This study is an exploratory study. Isolation of endophytic bacteria from sea holly leaves was carried out using the tissue section method on Nutrient Agar media. Antibacterial activity was tested using the well diffusion method. Characterization of endophytic bacteria was carried out based on phenotypic characters, namely colony and cell morphology characters, biochemical characters and physiological characters. A total of nine endophytic bacterial isolates were obtained from sea holly leaves. Based on the results of the antibacterial test, three endophytic bacterial isolates, namely isolate DM1.1, isolate DM1.2 and isolate DM2.3 were able to inhibit the growth of S. aureus and E. coli with a strong inhibition category. The characteristics of endophytic bacterial isolates DM1.1, DM1.2 and DM2.3 are rod-shaped cells (bacilli), Gram-positive, motile, catalase-negative, aerobic and able to grow at high salinity. Thus, endophytic bacteria from sea holly leaves have the potential to produce antibiotics.