Senyawa Prolin, Flavonoid dan Aktivitas Antioksidan Daun Tumbuhan Kamama (Litsea glutinosa (Lour.) C. B. Robins) di Desa Wadiabero Kecamatan Gu Wilayah Karst Kabupaten Buton Tengah

Abstract

This study aims to determine the levels of proline and flavonoid compounds and antioxidant activity of Kamama (Litsea glutinosa (Lour.) C. B. Robins) from Wadiabero Village, Gu District, Central Buton Regency. The type of exploratory research uses quantitative and qualitative methods. 600 grams of the simplicia were extracted using 96% ethanol followed by measurement of proline and flavonoid content using spectrophotometry. Antioxidant activity testing was conducted using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method. Data analysis was conducted using Microsoft Excel to obtain the linear regression equation in determining the levels of proline and flavonoid compounds and the IC₅₀ value. The result showed that the proline compound content of Kamama (Litsea glutinosa (Lour.) C. B. Robins) was 18,980 μmol/gram The flavonoid compound content was 2885 mg QE/g. The antioxidant activity of Kamama (Litsea glutinosa (Lour) C. B. Robins) has an IC₅₀ value of 74,391, indicating strong antioxidant activity.