Produksi Biofilm oleh Strain Klebsiella pneumoniae Resistan Antibiotik

Abstract

Klebsiella pneumoniae is known as a Gram-negative bacterium that most often causes infections and is resistant to antibiotics, making it very difficult to treat. Bacteria have several resistance mechanisms, one of which is biofilm production. The purpose of this study was to analyze the biofilm production of Klebsiella pneumoniae strains that are resistant to the antibiotic ciprofloxacin, as a form of defense mechanism. The in vitro research method was used to achieve the goal, carried out by testing clinical specimens of blood, sputum, pus, urine, feces, pleural fluid, with the process of isolation and identification, resistance testing and measuring biofilm production. The tested resistant K. pneumoniae isolates have a resistance mechanism by producing strong, medium and weak qualification biofilms, as well as low porin cell permeability. A strong significant correlation was formed in biofilm production with the amount of bacterial cell growth at the MIC value, which can increase cases of resistance prevalence.