Efek Ekstrak Daun Bayam Brasil (Alternanthera sissoo hort) terhadap Jumlah Limfosit dan Indeks Organ Limfoid Mencit Terinduksi CFA

Abstract

Brazilian spinach (Alternanthera sissoo hort) is a potential plant to be used as an immunomodulator because of its various content of phytochemicals and vitamins, especially vitamin E. This study aimed to determine the potential of Brazilian spinach leaf extract in modulating the number of lymphocytes, as well as lymphoid organ index in mice induced with Complete Freund Adjuvant (CFA). Brazilian spinach leaves were extracted by maceration method in ethanol solvent. Parameters measured from its leaves extract included phytochemical groups, total flavonoid levels and vitamin E. Balb/C male mice were employed as experimental animals to test the effect of Brazilian Spinach leaves extract to mice lymphocytes count, and thymus and spleen index. Mice were acclimatized for 7 days, then induced with CFA via subcutaneous, and given oral treatment for 7 days based on the test group, namely KS (healthy control), KN (negative control), KP (vitamin E 200 IU), and EEBB (spinach leaves extract with doses of 209.25 mg/g BW, 418.4 mg/g BW and 627.72 mg/g BW). The results showed that Brazilian spinach extract contained flavonoids, alkaloids, saponins, steroids, and tannins. Its total flavonoid content was 86.25 mg QE/g and its vitamin E level was 375.5 mg/100 g. In vivo tests showed leaves extract modulating effect on lymphocyte counts and thymus and spleen index. Dose of 627.72 mg/g BW was the best one in providing immunomodulatory effects in the form of lymphocytes number decrease in CFA-induced mice.